mRNA of expression of per 1 in mice bone marrow mesenchymal stem cells irradiated by red laser

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Important Dates

Conference:

Aug. 18-20, 2018

Full Paper Due: Jul. 20, 2018

Abstract Due: Jul. 20, 2018

Audience Registration Due:
Aug. 18, 2018

Presentations of The Int'l Symposium on Photonics and Optoelectronics (SOPO 2016)

● mRNA of expression of per 1 in mice bone marrow mesenchymal stem cells irradiated by red laser
Author(s)
Peng Fei
Affiliation(s)
Renmin Hospital of Wuhan University
KEYWORDS
mRNA, mesenchymal stem cells
ABSTRACT
A core group of circadian genes regulate the circadian rhythms in mammalian cells. However, the mammalian cellular circadian rhythm in photobiomodulation remains unknown. A lot of evidence has show that >20% of expressed mRNAs in bone stissues oscillate in a circadian manner. The aim of this paper is to investigate the mRNA expression of period 1 (per 1) in murine bone marrow-derived mesenchymal stem cells (BMSCs) which were irradiated by 635nm red laser light. The cells were seeded in 35mm tissue-culture dishes at a density of 8×104 cells/dish. and cultured in Dulbecco’s modiﬁed Eagle’s medium (DMEM). BMSCs were irradiated once by 635nm red light with radiation energies 0J/cm2, 1J/cm2, 4J/cm2. mRNA expression of per 1 via Semi-Quantitative Real-time RT-PCR at 0h, 6h, 12h, 18h, 24h. The genes displayed a oscillatory period of nearly 24 hours. And 635nm laser light changed the mRNA expression of per 1. We conclude that red light irradiation can effect the circadian rhythm of BMSCs.