The effect of 635nm red laser irradiation on proliferation of bone marrow stem cells

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Important Dates

Conference:

Aug. 18-20, 2018

Full Paper Due: Jul. 20, 2018

Abstract Due: Jul. 20, 2018

Audience Registration Due:
Aug. 18, 2018

Presentations of The Int'l Symposium on Photonics and Optoelectronics (SOPO 2016)

● The effect of 635nm red laser irradiation on proliferation of bone marrow stem cells
Author(s)
Peng Fei
Affiliation(s)
Renmin Hospital of Wuhan University
KEYWORDS
635nm red laser irradiation, bone marrow stem cells
ABSTRACT
Photobiomodulation effects of low-energy light irradiation on regeneration have been reported in skin , nerve, and skeletal muscle tissues and bone. Bone Mesenchymal stem cells (BMSCs) are de-rived from bone marrow, which exhibited a ﬁbroblast-like appearance, and could differentiate in vitro into different lineages. However, there is a a reciprocal relationship between growth and os-teogenic differentiation in MSCs. Therefore, it’s important to investigate the effect of Low-level light irradiation (LLLI) on BMSCs. The aim of our study was to investigate the proliferation effect of 638nm red laser light on bone marrow MSCs with or without osteogenic supplements. Bone mar-row was collected from the 4-week-old Sprague–Dawley rats femur and tibiae. MSCs with and without osteogenic supplements both were divided into three groups. A continuous 635nm wave-length red light diode laser (a power output of 960mW) was used in the study. The size of light spot was 35mm in diameter. Irradiation was performed every other day since the half of medium was changed to osteogenic differentiation media (ODM). The first irradiation day was set as 0 day. The duration of each irradiation for red light was calculated at 10 seconds for 1 J/cm2, 20 seconds for 2 J/cm2. Two of these groups were used as controls: MSCs incubated in DMEM without irradiation (control 1), MSCs incubated in ODM without irradiation (control 2). Cellular proliferation was evaluated by using WST-8. Cell viability was assessed with WST-8 kit at 2, 4, 6 and 8 days, respec-tively. At 4, 6 and 8 days, groups cultured with DMEM showed significantly higher viabilities than that in groups with ODM. In groups with DMEM, red light at all doses significantly stimulated cell viability as compared with the control 1. Groups irradiated at 1 and 2 J/cm2 had more effective pro-liferation on 4 (P＜0.01) and 6 days (P＜0.05), when compared with the control 1. In groups with ODM, control 2 and the irradiated groups showed similar proliferation speeds. In conclusion, we can find that red light can promote proliferation of MSCs cultured in normal media, and suppress proliferation of MSCs cultured in ODM.