Bioinspired fabrication of optical fiber SPR sensors for immunoassays using polydopamine-accelerated electroless plating

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Important Dates

Conference:

Aug. 18-20, 2018

Full Paper Due: Jul. 20, 2018

Abstract Due: Jul. 20, 2018

Audience Registration Due:
Aug. 18, 2018

Presentations of The Int'l Symposium on Photonics and Optoelectronics (SOPO 2016)

● Bioinspired fabrication of optical fiber SPR sensors for immunoassays using polydopamine-accelerated electroless plating
Author(s)
Se Shi
Affiliation(s)
Tianjin University
KEYWORDS
optical fiber SPR sensors, immunoassays
ABSTRACT
Fabrication of optical fiber surface plasmon resonance (SPR) sensors has always been a challenge. It requires some complex and special rotation devices1,2 in the vacuum evaporation or sputtering systems, ensuring that the deposition of gold films on the cylindrical optical fiber surface is as uniform as possible. Our group is devoted to the study of the fabrication and functional modification of the optical fiber SPR sensors based on the chemical metal deposition and bioinspired adhesion.3,4 In this report, we will focus on our recent works in the fabrications of SPR sensors, especially, the facile, rapid and effective method for the fabrication of optical fiber SPR sensors via polydopamine (PDA)-accelerated electroless plating (ELP)5. Bioinspired PDA coating was utilized as the versatile material for optic-fiber functionalization and the gold seeds adsorption of the ELP (Fig. 1). The fabricated sensors exhibited higher sensitivity, better reproducibility and adhesion stability compared with those fabricated by the traditional ELP. Some key experimental parameters, including DA polymerization temperature, DA polymerization time, and plating time, were investigated in detail. The optimized samples exhibited high sensitivity ranging from 1391 nm/RIU to 5346 nm/RIU in the refractive index range of 1.328 to 1.386. Scanning electron microscopy images indicated that the sensor surface consisted of gold nanoparticles with a uniform particle size and an orderly arrangement, and the film thickness was approximately 60 nm. Another PDA layer was formed on the gold film for the facile immobilization of antibodies. The sensor exhibited effective antibody immobilization ability and high sensitivity for human IgG detection over a wide range of concentrations from 0.5 to 40 μg/mL, which indicate the potential applications of fabricated sensors in immunoassays.